General Cell Based Assays
TRIBIOSCIENCE INC Mitochondrial Complex III Activity Colorimetric Assay 100 Tests
The activity of Complex III is directly measured through the increase of reduction of cytochrome c at absorbance of 550nm. This kit uses cytochrome c as substrate. The reduced cytochrome c is determined by recording the change in absorbance (A550). Applications Complex III Activity.
TRIBIOSCIENCE INC Cytochrome C Oxidase Activity Assay 100 Tests
The colorimetric assay in this kit is based on the observation of the decrease in absorbance at 550 nm of ferrocytochrome c caused by its oxidation to ferricytochrome c by cytochrome c oxidase. It is suitable for the detection of mitochondrial outer membrane integrity and for the detection of mitochondria in subcellular fractions.
Cayman Chemical 6-alkyn NAD sodIum salt 700ug
A clickable form of NAD+; has been used in conjunction with fluorescent tags in the detection of PARP protein substrates
Abcam Complex II Enzyme Activity Microplate Assay Kit
Complex II Enzyme Activity Microplate Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
TRIBIOSCIENCE INC Ethanol Assay Kit (Colorimetric / Fluorometric) 100 Tests
Tribioscience Ethanol Colorimetric Assay Kit provides a rapid, simple, reproducible, and sensitive tool for assay ethanol in plasma, serum, urine, and other bio-samples. The ethanol uses a single wording reagent to combine the alcohol oxidase reaction and color reaction into one step. The change in color intensity of the reaction product at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to ethanol concentration in the sample. The fluorometric assay is more sensitive than the colorimetric assay.
TRIBIOSCIENCE INC Cyclic GMP Colorimetric Activity Assay
This assay kit is a competition enzyme-linked immunoassay designed to measure cGMP levels in cells or tissues. An anti-rabbit IgG was pre-coated onto a microplate. The cGMP in test sample competes with cGMP-HRP for binding to an anti-cGMP rabbit monoclonal antibody. Following the incubation and wash step, an ultra-sensitive substrate TMB is added for color development. Because of the competitive nature of this assay, the color intensity is inversely proportional to the quantity of sample cGMP levels. Measurement of absorbance using the cGMP Standard allows calculating the absolute amount of cGMP in a sample. This assay provides a flexible, accurate, simple, and time-saving approach for cGMP detection.
TRIBIOSCIENCE INC Glycerol Colorimetric Assay 100 Tests
Tribioscience’s Glycerol Colorimetric Assay kit is a robust, simple, and high throughput method based on coupled enzyme reactions. Glycerol is phosphorylated and oxidized to generate hydrogen peroxide, which is catalyzed and reacts with the probe measured by the colorimetric method at OD = 570 nm.
Dojindo Molecular Technologies Inc Glutamate Assay Kit-WST (100 tests) is used to determine intracellular levels of Glutamate with easy to adjust dilutions for standard curve and high stability of stock solution 100 tests
Glutamate is not only used for the biosynthesis of proteins and glutathione but also plays an important function as a neurotransmitter. Excess of glutamate is believed to contribute to neurodegenerative diseases such as Alzheimer’s disease. A recent study showed that inhibition of cystine/glutamate transporter (xCT), which takes in cystine and releases glutamate, induces ferroptosis, and current cancer research is exploring xCT as a target. Glutamate Assay Kit-WST enables quantitation of glutamate as a metabolite. This kit has been optimized to quantify glutamate in cell culture supernatants and intracellular glutamate by measuring absorbance in a colorimetric WST reaction. This kit is formatted for 96-well microplates and the assay has a detection limit of 5 μmol/l glutamate. Dojindo has various quantification kits of Intracellular Metabolites. (https://www.dojindo.com/quantification-of-intracellular-metabolites/)
TRIBIOSCIENCE INC Neuraminidase Activity Fluorometric Assay 100 Tests
The Neuraminidase Activity Fluorometric Assay provides a simple, accurate, and sensitive method for monitoring neuraminidase activity in biological samples. In this assay, neuraminidase cleaves a non-fluorescent substrate to release a fluorescent product, which can be measured at an excitation wavelength of 365 nm and an emission wavelength of 445 nm. Applications - Neuraminidase activity in biological samples. Infuenza virus antigen titration. Key Features - Simplicity - The assay directly detects enzymatic activity without complex enzyme coupling steps. Sensitivity - Even low levels of neuraminidase activity can be detected due to the high fluorescence intensity.